chondrogenic media  (MedChemExpress)

Journal: Bioactive Materials

Article Title: Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D ☆

doi: 10.1016/j.bioactmat.2026.03.014

Figure Lengend Snippet: Increasing stress relaxation and plasticity in ASG accelerate MSC-based cartilage formation in 3D . Reverse transcription quantitative-polymerase chain reaction (RT-qPCR) analysis of chondrogenic markers for differentiation including A) transcription factor SRY-box transcription factor 9 ( SOX9 ) and cartilage matrix proteins B) aggrecan ( ACAN ) and C) type-2 collagen ( COL2 ) relative to SG (N = 3-4) and normalized to GAPDH. D) Safranin O staining of cryosectioned hydrogels at day 7 and day 21. Scale bar 75 μm. Quantification of E) day 7 and F) day 21 sulfated glycosaminoglycan (sGAG) content per hydrogel using a dimethylmethylene blue assay (DMMB) (N = 3). G) Immunohistochemical staining for type-2 collagen at day 28. Scale bar 300 μm ns, not significant; ∗ P < 0.05, ∗∗ P < 0.005, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001. The P values are obtained using one-way ANOVA with Tukey's multiple comparisons tests.

Article Snippet: The following compounds were added to chondrogenic media for the first three days of culture: 50 μM Blebbistatin (MedChem Express); 10 μM Y27632 (Sigma-Aldrich); and DMSO was also added to relevant control groups.

Techniques: Reverse Transcription, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Staining, Dimethylmethylene Blue Assay, Immunohistochemical staining

Journal: Bioactive Materials

Article Title: Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D ☆

doi: 10.1016/j.bioactmat.2026.03.014

Figure Lengend Snippet: Integrin β-1 expression and cytoskeletal tension correlate with MSC survival and chondrogenesis in ASG. A) Relative gene expression of integrin β-1 ( ITGB1 ) on day 1 (N = 3). B) Representative western blots for integrin β-1 at day 3 and C) quantification (N = 3). GAPDH is used as a loading control and was run on the same blot. D) Representative immunofluorescent images of z-projected integrin β-1 staining on day 3. Scale bar 5 μm. E) Quantification of relative fluorescent intensity of integrin β-1 staining (N = 15 cells across 3 hydrogels). F) Schematic of experimental timeline for drug studies. CM = chondrogenic media; bleb = blebbistatin. Drugs were administered for the first three days of culture in CM and samples were harvested at day 7 and day 21. G) Cell viability is evaluated using live/dead staining at day 7 and H) quantified as a percent viability (N = 3). Scale bar 100 μm. I) Safranin O staining of final tissue formation outcomes at day 21. Scale bar 100 μm ns, not significant; ∗ P < 0.05, ∗∗ P < 0.005, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001. The P value is obtained using an unpaired two-tailed t -test for A, C, and E and using a two-way ANOVA with Tukey's multiple comparisons test for H.

Article Snippet: The following compounds were added to chondrogenic media for the first three days of culture: 50 μM Blebbistatin (MedChem Express); 10 μM Y27632 (Sigma-Aldrich); and DMSO was also added to relevant control groups.

Techniques: Expressing, Gene Expression, Western Blot, Control, Staining, Two Tailed Test

Journal: Stem Cell Research & Therapy

Article Title: Immortalized human hair follicle-derived mesenchymal-like stromal cells for the long-term production of scalable Immunomodulatory and regenerative secretome

doi: 10.1186/s13287-025-04775-8

Figure Lengend Snippet: iHF-MSC immortalization and phenotyping. A SV40LT mRNA expression by RT-PCR. GAPDH was employed as a reference gene. Positive Control (C+): SV40T plasmid. Negative Control (C-): non-infected HF-MSCs. B Flow cytometry histograms for iHF-MSC surface markers pre-gated on live cells after doublet discrimination. Percentages shown correspond to the proportion of positive cells for the populations indicated in the legend (in order: isotype, HF-MSCs, C18, C20, C26, C39). Histograms shown are representative of one replicate. Y-axis: Count. C iHF-MSC differentiation ability. Optical micrographs of Alizarin Red S staining for osteogenic differentiation, Oil Red O staining for adipogenic differentiation, and Alcian Blue staining for chondrogenic differentiation. D Epifluorescence microscopy images of CD56 immunostaining (DAPI in blue, CD56 in green). Scale bars = 20 μm. Data are representative of 3 independent experiments with 4 replicates per group. Abbreviations: HF-MSCs: hair follicle-derived mesenchymal stromal cells. iHF-MSCs: immortalized HF-MSCs. C18: clone 18. C20: clone 20. C26: clone 26. C39: clone 39. CD: Cluster of Differentiation. MHC-II: major histocompatibility complex class II

Article Snippet: To do so, cells were seeded at a confluence of 2 × 10 5 cells per well in 96-well U-bottom suspension culture plates and incubated for 24–48 h. Spheroids were then cultured in chondrogenic differentiation media (Merck ready-to-use PromoCell MSC Differentiation Media: C-28012).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Positive Control, Plasmid Preparation, Negative Control, Infection, Flow Cytometry, Staining, Epifluorescence Microscopy, Immunostaining, Derivative Assay, Immunopeptidomics